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Detection of T1-oligonucleotides of the foot-and-mouth disease virus RNA [correction of DNA] by silver staining

Liebermann, H.; Fedtke, C.; Kwasniowski, W.

Acta Virologica 34(2): 202-205

1990

ISSN/ISBN: 0001-723X
PMID: 1698016
Document Number: 360486
Viral (v) RNA was isolated from purified foot-and-mouth disease virus (FMDV) by phenol-chloroform-isoamylalcohol treatment, digested by RNase T1 and separated by one-dimensional polyacrylamide gel electrophoresis (PAGE). The oligonucleotides were detected by silver staining. About 45 .mu.g of vRNA corresponding to about 100 ml of infectious BHK-21 cell culture fluid yielded a pattern of nearly 20 bands sufficient to differentiate between the FMD viruses.

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