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Preadsorption of boar semen with kaolin: increased efficiency of foot-and-mouth disease virus detection

Richmond, J.Y.

American Journal of Veterinary Research 39(10): 1612-1616

1978

ISSN/ISBN: 0002-9645
PMID: 213994
Document Number: 134576
Boar semen contains factors capable of inhibiting replication of FMD virus or masking its infectivity. Nonspecific antiviral and cytotoxic substances in semen are an additional complication for virus detection. The cytotoxic factor (but not the antiviral activity) were removed by adsorption with kaolin. Although FMD virus was efficiently removed from the medium by kaolin or kieselguhr, the virus was not removed from semen-virus mixtures. Because the cytotoxicity induced by boar semen apparently altered the ability of tissue culture cells to support virus replication, preadsorption with kaolin increased the probability of detecting this virus in semen samples.

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