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Relationship between internalization kinetics and cytotoxicity of mistletoe lectin I to L1210 leukaemia cells

Walzel, H.; Jonas, L.; Rosin, T.; Brock, J.

Folia Biologica 36(3-4): 181-188

1990

ISSN/ISBN: 0015-5500
PMID: 2257937
Document Number: 356360
We have taken two different approaches to the study of the entry of mistletoelectin I (MLI) into murine L1210 leukaemia cells. As detected by cellular protein synthesis and DNA synthesis inhibition, the lectin was cytotoxic to L1210 leukaemia cells. Inhibiton of [3H]leucine and [3H]thymidine incorporation into L1210 cells by MLI was found dose dependent in a concentration range from 10-16 to 10-12 mol/ml. The kinetics of cellular protein synthesis inhibition by MLI was concentration dependent, too. Using preembedding electron microscopy, the binding and intracellular routing of the gold-labelled lectin (MLI.Au15) were studied. MLI was internalized into L1210 leukaemia cells by two different pathways: via coated pits to coated vesicles and via long enclosed invaginations of the plasma membrane.

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Janssen, O.; Scheffler, A.; Kabelitz, D. 1993: In vitro effects of mistletoe extracts and mistletoe lectins. Cytotoxicity towards tumor cells due to the induction of programmed cell death (apoptosis) Arzneimittel-Forschung 43(11): 1221-1227
Metzner, G.; Franz, H.; Kindt, A.; Schumann, I.; Fahlbusch, B. 1987: Effects of lectin I from mistletoe (ML I) and its isolated A and B chains on human mononuclear cells: mitogenic activity and lymphokine release Die Pharmazie 42(5): 337-340
Gabius, H.J.; Walzel, H.; Joshi, S.S.; Kruip, J.; Kojima, S.; Gerke, V.; Kratzin, H.; Gabius, S. 1992: The immunomodulatory beta-galactoside-specific lectin from mistletoe: partial sequence analysis, cell and tissue binding, and impact on intracellular biosignalling of monocytic leukemia cells Anticancer Research 12(3): 669-675
Maliuchenko, N.V.; Moĭsenovich, M.M.; Egorova, S.G.; Gusarova, V.I.; Agapov, I.I.; Balashova, T.A.; Tonevitskiĭ, A.G. 1999: Analysis of major antigenic determinants in Mistletoe lectin i Biofizika 44(6): 1017-1021
Ziska, P.; Eifler, R.; Franz, H. 1979: Chemical modification studies on the D-galactopyranosyl binding lectin from the mistletoe Viscum album L Acta Biologica et Medica Germanica 38(9): 1361-1363
Bushueva, T.L.; Tonevitskiĭ, A.G.; Kindt, A.; Franz, H. 1988: The structure of toxic protein, the mistletoe lectin, at different pH: the study using intrinsic fluorescence method Molekuliarnaia Biologiia 22(3): 628-634
Beuth, J.; Ko, H.L.; Tunggal, L.; Buss, G.; Jeljaszewicz, J.; Steuer, M.K.; Pulverer, G. 1994: Immunoprotective activity of the galactoside-specific mistletoe lectin in cortisone-treated BALB/c-mice In Vivo 8(6): 989-992
Franz, H.; Kindt, A.; Ziska, P.; Bielka, H.; Benndorf, R.; Venker, L. 1982: The toxic A-chain of mistletoe lectin I: isolation and its effect on cell-free protein synthesis Acta Biologica et Medica Germanica 41(4): K9
Wagner, M.; Wagner, B.; Franz, H.; Ziska, P. 1978: Use of ferritin conjugates of a lectin from mistletoe (Viscum album L.) for the electron microscopic localization of cell surface receptors Acta Biologica et Medica Germanica 37(10): 1537-1542
Rostock, M.; Huber, R.; Greiner, T.; Fritz, P.; Scheer, R.; Schueler, J.; Fiebig, H.H. 2005: Anticancer activity of a lectin-rich mistletoe extract injected intratumorally into human pancreatic cancer xenografts Anticancer Research 25(3b): 1969-1975
Knöfler, W. 1985: The cytotoxicity of various metals with oxide layers in vitro. 1: Effect on the proliferation kinetics of monolayer cells Stomatologie der DDR 35(11): 633-637
Jondal, M.; Targan, S. 1978: Spontaneous and lectin-dependent cellular cytotoxicity by lymphocyte subpopulations against cell lines susceptible or resistant to spontaneous cytotoxicity Clinical and Experimental Immunology 33(1): 121-127
Van Oosterhout, Y.V.; van den Herik-Oudijk, I.E.; Wessels, H.M.; de Witte, T.; van de Winkel, J.G.; Preijers, F.W. 1994: Effect of isotype on internalization and cytotoxicity of CD19-ricin a immunotoxins Cancer Research 54(13): 3527-3532
Thilo, L. 1988: Kinetics of internalization and recycling of cell-surface glycoproteins Progress in Clinical and Biological Research 270: 377-390
Hurley, D.J.; Mastro, A.M. 1987: Differentiation of peanut lectin positive suppressor T-cells from peanut lectin negative precursors in bovine cells by 12-O-tetradecanoylphorbol-13-acetate Cancer Research 47(14): 3729-3735
Clementi, F.; Sher, E. 1985: Antibody induced internalization of acetylcholine nicotinic receptor: kinetics, mechanism and selectivity European Journal of Cell Biology 37: 220-228
Bradley, T.P.; Bonavida, B. 1982: Mechanism of cell-mediated cytotoxicity at the single cell level. V. the importance of target cell structure in cytotoxic T lymphocyte-mediated antigen nonspecific lectin-dependent cellular cytotoxicity Journal of Immunology 129(6): 2352-2356
Gutiérrez, F.; Garrido, F.; Osorio, C. 1979: Antibody-dependent cellular cytotoxicity in humans. Relationship between lymphoid cells with Fc receptors and ADCC Sangre 24(2): 135-142
Lang, N.P.; Ortaldo, J.R.; Bonnard, G.D.; Herberman, R.B. 1982: Interferon and prostaglandins: effects on human natural and lectin-induced cytotoxicity Journal of the National Cancer Institute 69(2): 339-343
Wöhrer, S.; Rabitsch, W.; Shehata, M.; Kondo, R.; Esterbauer, H.; Streubel, B.; Sillaber, C.; Raderer, M.; Jaeger, U.; Zielinski, C.; Valent, P. 2007: Mesenchymal stem cells in patients with chronic myelogenous leukaemia or bi-phenotypic Ph+ acute leukaemia are not related to the leukaemic clone Anticancer Research 27(6b): 3837-3841