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The toxic A-chain of mistletoe lectin I: isolation and its effect on cell-free protein synthesis

Franz, H.; Kindt, A.; Ziska, P.; Bielka, H.; Benndorf, R.; Venker, L.

Acta Biologica et Medica Germanica 41(4): K9

1982

ISSN/ISBN: 0001-5318
PMID: 7124246
Document Number: 194480
The reduction of Sepharose bound mistletoe lectin I (ML I) is a convenient method for preparing A- and B-chains under mild conditions. The B-chain represents the D-galactose binding moiety. The A-chain shows a significant dose-dependent inhibition of protein synthesis in cell-free systems from rat liver and rabbit reticulocytes, while pure B-chain preparations do not affect ribosomal translation processes.

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