Increase of calcium ion level in the cytoplasm of indo 1-loaded Hela cells under the action of histamine
Bochkov, V.N.; Cheglakov, I.B.; Ligum, D.E.; Gavrilov, I.I.; Avdonin, P.V.
Biulleten' Eksperimental'noi Biologii i Meditsiny 109(1): 40-42
1990
ISSN/ISBN: 0365-9615 PMID: 2159351 Document Number: 352808
The regulation of free cytoplasmic calcium in HeLa cells was studied using a fluorescent calcium indicator into 1. It is found that addition of histamine caused substantial increase in calcium level. In calcium-free medium the increase was much smaller, implicating both intra- and extracellular calcium as sources of observed Ca2(+)-rise. Protein kinase C activator, phorbol myristate acetate, completely blocked the effect of histamine. Agents causing rise in cellular cAMP level did not change the Ca2(+)-rise.