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Purification of transketolase from baker's yeast by an immunoadsorbent

Tikhomirova, N.K.; Kochetov, G.A.

Biochemistry International 22(1): 31-36

1990

ISSN/ISBN: 0158-5231
PMID: 2282080
Document Number: 351314
Baker's yeast transketolase has been purified by immunoaffinity chromatography on specific TK antibodies covalently linked to CNBr-agarose. Affinity chromatography allows a 480-fold purification of TK from yeast extracts and about 80% recovery of the original activity. The isolated enzyme has a specific activity of 12-60 U/mg and during polyacrylamide gel electrophoresis performed at pH 8.9 migrates as two protein bands possessing a transketolase activity which corresponds to two isoforms of the enzyme.

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Kochetov, G.A.; Chernov, N.N. 1970: Isoforms of transketolase from baker's yeast Biokhimiia 35(5): 1059-1061
Cherniak, V.I.; Beliaeva, R.K.; Magretova, N.N.; Kochetov, G.A. 1977: Reversible dissociation of transketolase from baker's yeast Biokhimiia 42(1): 159-161
Jin, T.; Kobayashi, T.; Honke, K.; Gasa, S.; Makita, A. 1992: Use of immunoadsorbent column chromatography for improved purification of arylsulfatase B from human placenta Biochemistry International 26(6): 1025-1033
Palagina, N.K.; Rozmanova, N.V.; Vitrinskaya, A.M.; Khrychova, A.I. 1975: Optimization of cultivation conditions of baker's yeast Prikladnaia Biokhimiia i Mikrobiologiia 11(4): 500-505
Lutskaia, A.I.; Kolpakov, A.I.; Kepecheva, R.M.; Ignat'eva, N.P.; Kupriianova-Ashina, F.G. 1998: The use of pancreatic RNAse in the production of baker's yeast Prikladnaia Biokhimiia i Mikrobiologiia 34(5): 588-591
Bocharova, N.N.; Chernysh, V.G.; Ozerova, V.P. 1973: Proteolysis in compressed baker's yeast during storage Prikladnaia Biokhimiia i Mikrobiologiia 9(4): 497-501
Heitmann, P.; Möllerke, C.; Uhlig, H.J. 1972: The state of tyrosine in inorganic pyrophosphatase of baker's yeast Acta Biologica et Medica Germanica 29(4): 551-560
Iscaki, M. 1971: Comparison of acetate and ethanol action on the oxidative degradation of glucose by baker's yeast Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 165(5): 1022-1026
Bienwald, B.; Höhne, W.E. 1978: Kinetic studies of the interaction of uranyl ions with inorganic pyrophosphatase from baker's yeast Acta Biologica et Medica Germanica 37(7): 1129-1133
Kuo, W.N.; McNabb, M.; Kanadia, R.N.; Dopson, N.; Morgan, R. 1997: The presence/absence of Bcl-2, Ca2+/calmodulin-dependent protein kinase IV, calretinin and p53 in baker's yeast and wheat germ Cytobios 91(364): 7-13
Mazo, V.K.; Gmoshinskiĭ, I.V.; Sokolova, A.G.; Zorin, S.N.; Danilina, L.L.; Litvinova, A.V.; Radchenko, S.N. 1999: Effect of biologically active food additives containing autolysate of baker's yeast and spirulina on intestinal permeability in an experiment Voprosy Pitaniia 68(1): 17-19
Golubkina, N.A.; Gmoshinskiĭ, I.V.; Zorin, S.N.; Danilina, L.L.; Chistiakov, A.V.; Mazo, V.K. 1998: Effect of a bioactive food supplement from selenium enriched baker's yeast autolysate on the status of the intestinal barrier in rats with anaphylaxis Voprosy Pitaniia 1998(3): 18-22
Li, Y.; Chen, J.; Song, Q.; Lun, S.; Katakura, Y. 1997: Fed-batch culture strategy for high yield of baker's yeast with high fermentative activity Chinese Journal of Biotechnology 13(2): 105-113
Uritani, M.; Iwasawa, J. 1993: Purification and characterization of RNases from fission yeast under nitrogen-starvation Nucleic Acids Symposium Series 29: 129-130
Bystrykh, L.V.; Trotsenko, I.A. 1983: Purification and several properties of dihydroxyacetone kinase from the methylotrophic yeast Candida boidinii Biokhimiia 48(10): 1611-1616
Ottonello, S.; Ballabeni, A.; Soncini, C.; Dieci, G. 1994: High level expression in E. coli and purification of yeast transcription factor IIIA Biochemical and Biophysical Research Communications 203(2): 1217-1223
Ivanov, P. 1977: Use of an immunoadsorbent in differentiating B and D salmonelloses in poultry Veterinarno-Meditsinski Nauki 14(9): 28-32
Jaros-Kamińska, B.; Opacka, J.; Slaska, K.; Ciesielska, E.; Szmigiero, L.; Gniazdowski, M. 1977: Attempts at transcription of yeast nuclear and mitochondrial DNA. II. Template activity of yeast DNA Medycyna Doswiadczalna i Mikrobiologia 29(1): 35-42
De Bortoli, M.; Roggero, P. 1985: Electrophoretic desorption of intact virus from immunoadsorbent Microbiologica 8(2): 113-121
Hossein, M.; Mirhendi, S.H.; Brandão, J.ão.; Mirdashti, R.; Rosado, L. 2011: Comparison of Enzymatic Method Rapid Yeast Plus System with RFLP-PCR for Identification of Isolated Yeast from Vulvovaginal Candidiasis Iranian Journal of Basic Medical Sciences 14(5): 443-450