Kinetic studies of the interaction of uranyl ions with inorganic pyrophosphatase from baker's yeast
Bienwald, B.; Höhne, W.E.
Acta Biologica et Medica Germanica 37(7): 1129-1133
1978
ISSN/ISBN: 0001-5318 PMID: 218410 Document Number: 128001
Kinetic measurements were performed to test the effect of uranyl ions on the enzymatic hydrolysis of pyrophosphate. A strong inhibition of the enzyme was found. From a Dixon-plot an inhibition competitive to the substrate magnesium pyrophosphate and an inhibitory constant of Ki = 3 . 10(-7) M was deduced.