Immunoenzyme analysis of insulin antibodies in human blood serum
Sorokina, N.V.; Gavrilova, E.M.; Egorov, A.M.; Gitel', E.P.; Bol'shakova, T.D.
Laboratornoe Delo 2: 25-28
1990
ISSN/ISBN: 0023-6748 PMID: 1692361 Document Number: 350027
Indirect solid-phase enzyme immunoassay (EIA) was used for the titration of antibodies to insulin in human blood sera. The modification suggested by the authors permits assessing the immunoglobulins G and M insulin-binding ability. The technique was optimized for all the stages of analysis and a method for estimating the insulin-binding ability of IgG in the sera diluted 1:80 and of IgM in the sera diluted 1:320 was developed. Fifty blood sera of patients with insulin-dependent diabetes mellitus were analyzed. EIA results were in good correlation with the findings of radioimmunoassay.