Serological analysis of human IgG and IgE anti-insulin antibodies by solid-phase radioimmunoassays
Hamilton, R.G.; Rendell, M.; Adkinson, N.F.
Journal of Laboratory and Clinical Medicine 96(6): 1022-1036
1980
ISSN/ISBN: 0022-2143 PMID: 7000933 Document Number: 163811
A single solid-phase assay system which is useful for quantitative measurement of IgG and IgE anti-insulin antibodies in human serum was developed. Insulin-specific Ig are absorbed from human serum by excess quantities of insulin-agarose. After washes to remove unbound Ig, radioiodinated Staphylococcus aureus (Staph) A or rabbit anti-human IgE is added to detect bound IgG or IgE antibodies, respectively. Both assays demonstrate good performance characteristics as follows: sensitivity, 2.5 ng/ml (IgG) and 0.5 ng/ml (IgE); intra-assay precision (CV), 4.5% (IgG; n = 18) and 5.6% (IgE; n = 20); interassay reproducibility (CV), 7.2% (IgG; n = 6) and 8.1% (IgE; n = 18). IgG and IgE reference sera were standardized in weight/volume terms by solid-phase methods. Although SPRIA.