Quantitative determination of immunoglobulin a in human serum by an immunoenzyme technic
Dzantiev, B.B.; Gavrilova, E.M.; Egorov, A.M.; Smirnova, M.B.; Berezin, I.V.
Voprosy Meditsinskoi Khimii 24(5): 702-708
1978
ISSN/ISBN: 0042-8809 PMID: 360611 Document Number: 133233
A complex of immunoglobulin A (IgA) with peroxidase was obtained as a result of interaction of IgA with the enzyme, modified with sodium periodate. The molar ratio of IgA/peroxidase was equal to 1 in the complex, as shown by gelfiltration and sedimentation analysis. Peroxidase maintained 75% of the initial activity in the complex. The antigenic and enzymatic properties of the complex were retained completely within 5 months, if the preparation was kept at 4 degrees in phosphate buffer, pH 7.4. The complex isolated was used for quantitative estimation of IgA in human blood serum. The method is based on the reaction of competitive binding between free and labelled with peroxidase IgA molecules and antibodies, immobilized on inorganic porous carrier--sylochome. The method enables to estimate quantitatively IgA concentration within the limits of 102=105 ng/ml.