High resolution electrophoresis of urinary proteins from unconcentrated samples with silver staining
Shen, X.; Baumstummler, D.; Cloppet, H.; Richard, M.
Journal de Pharmacie de Belgique 44(2): 133-136
1989
ISSN/ISBN: 0047-2166 PMID: 2476542 Document Number: 344784
We have evaluated an electrophoretic technique on agarose gel with silver staining on one hundred pathological urine samples. This method allows to study low proteinuria and to characterize glomerular, tubular electrophoretic patterns and also to detect monoclonal immunoglobulin light chains.
Document emailed within 1 workday
Related Documents
Okon, E.B.; Marjanovic, V.; Hranisavljevic, J.; Vucelic, D. 1996: Silver staining of proteins separated by polyacrylamide gel electrophoresis: mathematical model Biokhimiia 61(12): 2082-2091Trbojević-Cepe, M.; Poljaković, Z.; Franjić, J.; Bielen, I.; Vranes, Z. 1989: Detection of oligoclonal IgG bands in unconcentrated CSF in multiple sclerosis and other neurological diseases by isoelectric focusing on ultrathin-layer polyacrylamide gel immunofixation and silver staining Neurologija 38(1): 11-21
Zardoya, R.; Diez, A.; Mason, P.J.; Luzzatto, L.; Garrido-Pertierra, A.; Bautista, J.M. 1994: High resolution of proteins by double-inverted gradient polyacrylamide gel electrophoresis (DG-PAGE) Biotechniques 16(2): 270
Patton, W.F.; Lopez, M.F.; Barry, P.; Skea, W.M. 1992: A mechanically strong matrix for protein electrophoresis with enhanced silver staining properties Biotechniques 12(4): 580-585
Morozov, V.A.; Il'inskiĭ, P.O.; Il'in, K.V. 1986: Advantages and disadvantages of silver staining of proteins in polyacrylamide gel Biulleten' Eksperimental'noi Biologii i Meditsiny 102(11): 620-623
Irie, S. 1980: A highly sensitive silver staining for detection of proteins in polyacrylamide gels Seikagaku. Journal of Japanese Biochemical Society 52(6): 411-413
Zhu, Z.; Li, L.; Zhu, Y. 1996: Detection of p53 gene mutation in bronchial biopsy samples from lung cancer patients with polymerase chain reaction-single strand conformation polymorphism-silver staining method Zhonghua Nei Ke Za Zhi 35(11): 738-740
Levy, N.; Navarro, A.; Moretti, C.; Barthelemy, M.; Stahl, A. 1996: The distribution of Ag-NORs proteins and ribosomal RNA genes in human spermatogonia: an original procedure associating silver-staining, fish and confocal microscopy Cellular and Molecular Biology 42(2): 159-168
Furr, M.; Chickering, W.R.; Robertson, J. 1997: High resolution protein electrophoresis of equine cerebrospinal fluid American Journal of Veterinary Research 58(9): 939-941
Darconza, G.; Paradiso, S.; De Michele, G.P. 1992: High resolution electrophoresis of serum aand amniotic fluid Minerva Ginecologica 44(10): 485-489
Contractor, B.Z.; Chakravorti, B.P. 1974: A profile of urinary proteins by disc-electrophoresis Indian Journal of Medical Sciences 28(10): 452-454
Marshall, T.; Williams, K.M.; Holmquist, L.; Carlson, L.A.; Vesterberg, O. 1985: Plasma apolipoprotein pattern in fish-eye disease examined by high-resolution two-dimensional electrophoresis Clinical Chemistry 31(12): 2032-2035
Aveynier, E.; Peronnon, C.; Valenti, K.; Laporte, F. 1998: Apolipoprotein (a) isoform size determination. Value and limits of high resolution phenotyping by agarose gel electrophoresis Annales de Biologie Clinique 56(1): 73-78
Bilobrov, V.M.; Mironov, O.L.; Khil'ko, S.B. 1987: Method of disk electrophoresis of urinary proteins on polyacrylamide gel Laboratornoe Delo 9: 678-680
Hochstrasser, D.; Augsburger, V.; Pun, T.; Weber, D.; Pellegrini, C.; Muller, A.F. 1988: "High-resolution" mini-two-dimensional gel electrophoresis automatically run and stained in less than 6 h with small, ready-to-use slab gels Clinical Chemistry 34(1): 166-170
Cachera, C.; Mizon, C.; Fruchart, J.C.; Mizon, J.; Tacquet, A. 1980: Rapid sodium dodecyl sulfat/polyacrylamide gel electrophoresis of urinary proteins Clinical Chemistry 26(11): 1588-1590
Koene, R.A.; Bogman, M.J.; van der Snoek, B.E. 1992: Distinction between renal and nonrenal hematuria using immunoperoxidase staining of urinary erythrocytes for Tamm-Horsfall proteins Nederlands Tijdschrift Voor Geneeskunde 136(44): 2198-2199
Rautenstrauch, H. 1977: Analysis of urinary proteins by disc electrophoresis: a method for the differential diagnosis of kidney diseases Journal of Clinical Chemistry and Clinical Biochemistry 15(6): 333-337
Yisheng, L.; Wenping, D.; Kuiyang, Z.; Linlin, F.; Ming, C. 2005: Comparative study on rapid dot-immunogold staining and two immunogold silver staining assays for diagnosing schistosomiasis japonica Southeast Asian Journal of Tropical Medicine and Public Health 36(1): 79-82
Van Steenkiste, G.; Poot, D.H.J.; Jeurissen, B.; den Dekker, A.J.; Vanhevel, F.; Parizel, P.M.; Sijbers, J. 2017: Super-resolution T1 estimation: Quantitative high resolution T1 mapping from a set of low resolution T1 -weighted images with different slice orientations Magnetic Resonance in Medicine 77(5): 1818-1830