Advantages and disadvantages of silver staining of proteins in polyacrylamide gel
Morozov, V.A.; Il'inskiĭ, P.O.; Il'in, K.V.
Biulleten' Eksperimental'noi Biologii i Meditsiny 102(11): 620-623
1986
ISSN/ISBN: 0365-9615 PMID: 2430644 Document Number: 268781
Sensitivity of protein staining with Serva blue G-250 (Coomassie brilliant blue G-250 analogue) in polyacrylamide gel was determined. It has been shown that protein staining with 0.1% Serva blue G-250 results in the recovery of 80 to 35 ng of single protein, that is almost 10 times higher than reported previously for Coomassie brill. blue G-250 (or R-250) staining. The comparison of the sensitivity of Serva blue G-250 protein staining in PAAG and AgNO3 has shown that AgNO3 staining was approximately 18-30 (but not 100 times, as it had been thought before) times more effective for the majority of proteins under study. Silver staining of some proteins, for instance ribonuclease and a number of retrovirus-specific structural proteins, was of lower efficacy. Thus, to obtain reliable results protein electrophoresis in PAAG should be followed by both staining procedures.