A simple method of protein determination in polyacrylamide gel using silver staining

Grebinozhko, E.I.; Nikolaenko, A.N.; Ivanov, A.P.

Ukrainskii Biokhimicheskii Zhurnal 58(5): 62-65

1986


ISSN/ISBN: 0201-8470
PMID: 2430353
Document Number: 277680
A simple and relatively rapid method is elaborated for development of electrophoregram in PAAG by silver binding directly with protein. This permits avoiding the gel staining, considerably improves the quality of staining and promotes better reproducibility of the results. Sensitivity of the method permits detecting 1 ng of protein in the band.

Document emailed within 1 workday
Secure & encrypted payments