Method of detection of protein kinase activity in polyacrylamide gel using two-dimensional protein separating system
Kit, I.I.; Koval'ova, V.A.; Bobak, I.P.; Drobot, L.B.
Ukrains'kyi Biokhimichnyi Zhurnal 74(4): 129-132
2002
ISSN/ISBN: 0372-3909 PMID: 14964875 Document Number: 548011
Method for detection of protein kinase activity in polyacrylamide gel have been developed. After separation of proteins by isoelectric focusing in non-denaturing condition, gel was incubated in a reaction buffer containing [gamma-32P]ATP. 32P-labeled proteins were separated by subsequent SDS/PAGE electrophoresis in second dimension. The proposed method was used for detection of protein kinase activity in human blood serum and triton X-100 soluble proteins of heads of Drosophila melanogaster.
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