The development of a method of nitrosoguanidine inactivation in the mutagenic treatment of bacteria
Kulichenko, A.N.; Popov, I.A.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 8: 5-7
1989
ISSN/ISBN: 0372-9311 PMID: 2686303 Document Number: 344783
A simple method for the inactivation of nitrosoguanidine, a mutagenic agent permitting the simplification and acceleration of the procedure used for obtaining bacterial mutants, is described. This method is based on the addition of unithiol to nitrosoguanidine solution to a final concentration of 0.25-0.5% and the subsequent normalization of the pH of the medium with 0.1 N NaOH solution.
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