Principles of selective inactivation of the viral genome. III. Kinetics of the inactivation of bacteriophage MS2 infectivity by beta-propiolactone
Budovskiĭ, E.I.; Zalesskaia, M.A.
Molekuliarnaia Biologiia 19(4): 1139-1147
1985
ISSN/ISBN: 0026-8984 PMID: 4047036 Document Number: 242226
It is shown that the action of beta-propiolactone on the bacteriophage MS2 under the wide range of conditions (pH, temperature, initial concentration of the reagent) the survival curves could be accurately described, taking into account the reagent consumption during inactivation. The requirements for accurate description of the infectivity inactivation of viruses by the action of any chemical agent were formulated. That allowed rational development of a principal stage in preparation of the killed antiviral vaccines--inactivation of the virus infectivity to the necessary extent by the action of chemical agents.