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Heat inactivation kinetics of solubilized and immobilized immunoglobulins G

Markina, V.L.; Eremin, A.N.; Metelitsa, D.I.

Prikladnaia Biokhimiia i Mikrobiologiia 29(1): 115-124

1993

ISSN/ISBN: 0555-1099
PMID: 8475016
Document Number: 407326
The kinetics of thermoinactivation of rabbit antibodies against cortisol (Anti-COR, 60-82 degrees), sheep antibodies against rabbit immunoglobulins (AB, 65-77 degrees) in water solution and of AB covalently linked to activated polystyrene beads (PSB-AB, 60-77 degrees) was studied by indirect and direct ELISA methods using peroxidase-cortisol or peroxidase-rabbit immunoglobulin conjugates to detect immunocomplexes for PSB-AB. High stability of ABs and their stabilization after immobilization on PSB are shown. The Eact values and thermodynamic activation parameters of immunoglobulins thermolysis delta H*, delta S* and delta G* were calculated from Arrhenius plots of the effective inactivation rate constants versus temperature and compared with the corresponding characteristics for other proteins.

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