The host cell fraction that increases the infectivity of bacteriophage f2

Chroboczek, J.

Acta Biochimica Polonica 25(1): 37-47

1978


ISSN/ISBN: 0001-527X
PMID: 352078
Document Number: 134164
A fraction that increases infectivity of bacteriophage f2 was isolated from uninfected E. coli cells. The greatest effect was obtained when the fraction was added to the phage reconstituted in vitro. The fraction isolated from the ribosome-free supernatant consisted of proteins, lipids, carbohydrates, and unidentified material. Cleavage of protein by the treatment with trypsin did not significantly affect the infectivity-restoring activity. It is suggest that lipids may play an essential role in the activity of the fraction isolated from the host cell.

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