Regulation of podosomes by intracellular pH in avian osteoclasts
Teti, A.; Grano, M.; Teitelbaum, S.L.; Hruska, K.A.; Colucci, S.; Zambonin Zallone, A.
Bollettino della Societa Italiana di Biologia Sperimentale 65(7): 597-601
1989
ISSN/ISBN: 0037-8771 PMID: 2597415 Document Number: 342283
The effects of changes in intracellular pH (pH1) on the organization of the clear zone of isolated avian osteoclasts in culture were studied. The distribution of podosomes, the close contact areas that mediate the adhesion of osteoclasts to the substrate, was investigated by decoration of microfilaments with fluorescent phalloidin. Intracellular acidification by butyric acid induces significant increase of podosome formation at the level of the clear zone compared to controls. Conversely, alkalinization by HCO3- reduces the percentage of osteoclasts with podosomes. A role of pH1 on the adhesion of the osteoclasts to the substrate is hypothesized.