Thyroid status and regulation of intracellular sodium in rabbit heart
Doohan, M.M.; Gray, D.F.; Hool, L.C.; Robinson, B.G.; Rasmussen, H.H.
American Journal of Physiology 272(4 Pt 2): H1589-H1597
1997
ISSN/ISBN: 0002-9513 PMID: 9139940 Document Number: 480747
To examine the effect of thyroid status on the homeostatic control of intracellular Na+, we studied the effect of treatment of hypothyroid rabbits with 3,5,3'-triiodothyronine (T-3). Intracellular Na+ and pH (pH-i) in papillary muscles and Na+-K+ pump current (I-p) in ventricular myocytes were measured with ion-sensitive microelectrode and whole cell patch-clamp techniques. Na+ influx, estimated from the rate of increase in intracellular Na+ on sudden Na+-K+ pump blockade with dihydroouabain, and Na+ efflux, calculated from I-p, were similar. Treatment with T-3 induced an increase in both Na+ influx and I-p. The treatment-induced increase in Na+ influx was eliminated by 5-(N,N-dimethyl)amiloride (DMA) but not by tetrodotoxin. Treatment with T-3 increased the rate of fall in pH-i on exposure of the papillary muscles to DMA; when the buffer capacity was taken into account, the T-3 treatment-induced increase in this rate corresponded well with the treatment-induced, DMA-inhibitable estimate of Na+ uptake. We conclude that thyroid hormone enhances both Na+-H+ exchange-mediated Na+ uptake and Na+-K+ pump-mediated Na+ efflux.