Influence of surface pH on intracellular pH regulation in cardiac and skeletal muscle
Vanheel, B.; de Hemptinne, A.; Leusen, I.
American Journal of Physiology 250(5 Pt 1): C748-C760
1986
ISSN/ISBN: 0002-9513 PMID: 3085512 Document Number: 279727
The influence of the surface pH (pHs) on the intracellular pH (pHi) and the recovery of pHi after an imposed intracellular acid load was investigated in isolated sheep cardiac Purkinje fiber, rabbit papillary muscle, and mouse and rat soleus muscle. pHs and pHi, respectively, were continuously measured by use of single- and double-barreled pH-sensitive glass microelectrodes. Surface acidosis, usually obtained by superfusion with solutions of acid pH, was also produced with low buffered (5 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid) solutions at control pH. The pHs decrease (.DELTA.pHs) induced by low buffering was smallest (-0.08 pH unit) in Purkinje fiber and largest (-0.31 pH unit) in rat soleus muscle, which already had a more acid surface in control conditions. .DELTA.pHs was somewhat dependent on the superfusion rate. Higher superfusion rates decreased but did not abolish .DELTA.pHs. Surface acidosis was associated with a small intracellular acidification. Intracellular acid loads were produced by adding and subsequently withdrawing 20 meq/l NH4+ from the superfusate. In all preparations, the rate of recovery of pHi after NH4+ withdrawal was notably decreased at acidified pHs. This effect was amiloride sensitive. It is concluded that, in superfused multicellular preparations, pHs and therefore the buffer concentration of a superfusate can considerably influence steady-state pHi and pHi recovery from an imposed intracellular acid load.