Intracellular acidification induces decrease of cytosolic calcium in isolated osteoclasts
Teti, A.; Grano, M.; Teitelbaum, S.L.; Hruska, K.A.; Colucci, S.; Zambonin Zallone, A.
Bollettino della Societa Italiana di Biologia Sperimentale 65(8): 689-693
1989
ISSN/ISBN: 0037-8771 PMID: 2803732 Document Number: 327448
In this study the effects of changes in intracellular pH on cytosolic Ca2+ were examined in single isolated osteoclasts. Alkalinization, performed by incubation in HCO3 containing buffer, induced increases in [Ca2+]i. Conversely acidification, obtained by incubation in Na-butyrate-containing buffer induced a rapid and sustained decrease of [Ca2+]i. The decrease of [Ca2+]i during acidification with Na-butyrate was inhibited by VO4, an inhibitor of the Ca2+-ATPase. All these pieces of information indicate that changes in pHi can modulate osteoclast activity together with modifications of [Ca2+]i, probably acting as intracellular signals.