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Calcium dependent adhesion structures in osteoclasts

Teti, A.; Carano, A.; Di Cillo, M.; Giancaspro, M.; Santacroce, G.; Zambonin Zallone, A.

Bollettino della Societa Italiana di Biologia Sperimentale 63(7): 589-596

1987

ISSN/ISBN: 0037-8771
PMID: 3663363
Document Number: 286367
The role of calcium in the regulation of osteoclast adhesion in vitro has been studied by fluorescence microscopy, using rodhamine-conjugated phalloidin to detect F-actin. Osteoclasts adhere in vitro on glass or plastic substrates by peculiar close-contact areas, called prodomes, containing gelsolin, a calcium dependent actin fragmenting protein which, at intracellular calcium concentration of uM, depolimerizes microfilaments. The results of the experiment showed that decreasing extracellular calcium concentration by culturing the osteoclasts in low calcium medium, the number of cells containing podosomes increased significantly. Treating osteoclasts with sodium orthovanadate, that blocks the calcium-ATPase and increases the intracellular the intracellular calcium, podosomes disappeared completely within 60 minutes, while control fibroblasts, that do not contain gelsolin or other calcium dependent actin regulating proteins, were not affected by the treatment.

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