Role of calcium ions in stabilizing rat liver adhesion factor
Iamskova, V.P.
Biofizika 23(3): 428-431
1978
ISSN/ISBN: 0006-3029 PMID: 667144 Document Number: 132417
Highly purified native preparation of adhesion factor (AF) from rat liver was shown to inactivate after dialysis against deionised water or after action of chelating agent (EGTA). Isoelectric point of inactivated AF was less than 2.0 Rf value in PAG in presence of SDS was significantly increased. The results obtained suggest that inactivation of AF during electrofucusing depends on ampholyne binding of Ca(II) which may be constituent in AF.