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The effect of bleomycin on DNA synthesis in human cells: evidence for grouping of initiation of replicons in the S-period

Liapunova, N.A.; Lavrushina, O.M.; Terekhov, S.M.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 1: 34-39

1989

ISSN/ISBN: 0208-0613
PMID: 2471926
Document Number: 342179
The effect of bleomycin (Blm) on DNA synthesis has been studied in a synchronous culture of human embryonic lung cells. The data obtained suggest that in the Blm presence in a medium (20 micrograms/ml) DNA synthesis initiation in new replicons is suppressed. The Blm action at different S-phase intervals has been shown to inhibit DNA synthesis unequally. Four discrete time intervals have been singled out in the course of the 10-hr S-phase in which a grouped initiation of replicon portions can be supposed. Together with the data on DNA replication in large-size replicon units (50-500 microns), the obtained results account well for the uneven DNA synthesis in S-phase, manifested by 3 or 4 peaks of [3H]-thymidine incorporation in pulse-labelled cells.

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