Effect of heat shock on protein synthesis by normal and malignant human lung cells in tissue culture
Tsukeda, H.; Maekawa, H.; Izumi, S.; Nitta, K.
Cancer Research 41(12 Pt 1): 5188-5192
1981
ISSN/ISBN: 0008-5472 PMID: 7307014 Document Number: 180058
Heat shock proteins were found in cultured human lung cells by sodium dodecyl sulfate:gel electrophoresis and autoradiography using [35S]methionine. The synthesis of a MW 70,000 protein was markedly stimulated in normal [HAIN-6 cells], malignant [squamous carcinoma PC-1 cells] and SV40-transformed [WI-26-Va4] cells, and that of MW 90,000 and MW 100,000 proteins was stimulated only in malignant and SV40-transformed cells after heat shock treatment. In contrast, the synthesis of MW 200,000 and MW 250,000 proteins observed in the unheated normal cells was diminished after the same procedure. The heat shock proteins were induced within a given range of temperature and duration of treatment, the conditions for normal and malignant cells being clearly different, i.e., optima of 43.degree. for 1 h in the normal cells and 41.degree. for 1 h in the malignant cells. The analysis of heat shock protein is probably very useful for identifying the differential heat susceptibilities of normal and malignant cells and for elucidating their bases and mechanisms. [Research has application to the hyperthermia therapy of tumors.].