Affinity labeling of ACTH receptors in bovine adrenal cortex membranes
Mizuno, T.; Okada, M.; Itoh, A.; Maruyama, T.; Hagiwara, H.; Hirose, S.
Biochemistry International 19(4): 695-700
1989
ISSN/ISBN: 0158-5231 PMID: 2559726 Document Number: 341860
Adrenocorticotropin labeled with 125I at Tyr23 ([125I-Tyr23]ACTH) was prepared by radioiodination of ACTH1-39 followed by reverse phase HPLC purification. When incubated with bovine adrenal cortical membranes, the radioligand bound specifically to a 40-kDa membrane protein as revealed by affinity labeling. This result indicates that the bovine adrenal ACTH receptor, whose identification and characterization have proved difficult, has an Mr of about 40,000.