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Purification of the pyruvate dehydrogenase complex from bovine adrenal cortex mitochondria

Strumilo, S.A.; Senkevich, S.B.; Vinogradov, V.V.

Biokhimiia 45(5): 883-888

1980

ISSN/ISBN: 0320-9725
PMID: 7378507
Document Number: 163103
Isolation of the pyruvate dehydrogenase complex from bovine adrenal cortex and its purification including fractionation by polyethyleneglycol, ultracentrifugation and gel filtration on Sepharose 4B is described. The preparation obtained having the specific activity of 4,5 U/mg was purified 370-fold with a yield of 37%. Under polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate the pyruvate dehydrogenase complex is degraded into 4 protein fractions with the mobility corresponding to the molecular weights of 74 000, 56 000, 42 000 and 37 000.

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Strumilo, S.A.; Taranda, N.I.; Senkevich, S.B.; Vinogradov, V.V. 1981: 2-oxoglutarate dehydrogenase complex from bovine-adrenal-cortex mitochondria. Purification and partial characterization Acta Biologica et Medica Germanica 40(3): 257-264
Strumilo, S.A.; Senkevich, S.B.; Vinogradov, V.V. 1981: Characterization of the kinase activity of bovine adrenal pyruvate dehydrogenase complex Biokhimiia 46(6): 974-978
Taranda, N.I.; Vinogradov, V.V.; Strumilo, S.A. 1987: Purification and properties of NADP-dependent isocitrate dehydrogenase from the bovine adrenal cortex Ukrainskii Biokhimicheskii Zhurnal 59(1): 24-29
Vinogradov, V.V.; Iarotskiĭ, I.V.; Zavodnik, I.B. 1992: Inhibition of pyruvate kinase of bovine adrenal cortex by ATP: the role of magnesium Biokhimiia 57(1): 91-96
Strumilo, S.A. 1983: Different efficiency of Mn2+, Ca2+ and Mg2+ ions, in the reaction of the pyruvate dehydrogenase complex of adrenal glands with thiamine pyrophosphate Voprosy Meditsinskoi Khimii 29(1): 94-97
Senkevich, S.B.; Strumilo, S.A.; Vinogradov, V.V. 1986: NADP-dependent malate dehydrogenase from bovine adrenal cortex cytoplasm. Isolation and properties Biokhimiia 51(6): 1023-1028
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