Direct oxidation of glutamate by mitochondria from porcine adrenal cortex
Popinigis, J.; Antosiewicz, J.; Mazzanti, L.; Bertoli, E.; Lenaz, G.; Cambria, A.
Biochemistry International 21(3): 441-451
1990
ISSN/ISBN: 0158-5231 PMID: 1977389 Document Number: 350937
Mitochondria isolated from porcine adrenal cortex under State 3 conditions oxidized succinate with a rate of 47 .+-. 4.48 na oxygen/min/mg/protein and with the ADP:0 ratio 0.98 .+-. 0.09. In the presence of 15 .mu.M deoxycorticosterone the rate of succinate oxidation was 36.8 .+-. 3.08 na oxygen/min/mg/protein. Under the same conditions the rate of glutamate oxidation was 22.8 .+-. 2.21 and 16.8 .+-. 0.65 na oxygen/min/mg/protein, respectively. The ADP:0 ratio was 1.45 .+-. 0.14. Introduction of trace amounts of malate into the mitochondria oxidizing glutamate only slightly increased the rate of 02 uptake. The glutamate dehydrogenase activity in these mitochondria was 12.5 .+-. 0.69 nmol/min/mg.