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Trypanosoma cruzi: conditions required to improve metacyclic differentiation in axenic culture

Isola, E.L.; Lammel, E.M.; Redruello, M.

Revista Argentina de Microbiologia 21(1): 9-14

1989

ISSN/ISBN: 0325-7541
PMID: 2664877
Document Number: 338906
This work is a study on the behaviour of Epi from various T. cruzi strains and clones grown in media stimulating differentiation such as M 16, TAUP, TAUS, LIT-hemin and G-IH. Results showed that in our experimental conditions the G-IH medium was the only suitable one to induce Epi-Mtc morphogenesis. Optimal temperature for both multiplication and differentiation in this medium was 28 degrees C. The use of G-IH medium after nutrient depletion by dilution failed to induce differentiation. Parasites obtained from biphasic medium at 24-48 h culture proved the best to achieve morphogenesis. External Ca++ supply blockage affected the differentiation capacity of Tul and RA strains to a variable degree.

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