Immunohistochemical studies of insulin receptors in the liver
Babichenko, I.I.
Biulleten' Eksperimental'noi Biologii i Meditsiny 108(10): 495-499
1989
ISSN/ISBN: 0365-9615 PMID: 2688753 Document Number: 338217
The indirect peroxidase-labeled antibody method was used to localize Insulin Receptors (IR) in rat livers. For experiments the rabbit antisera against synthetic peptides correspond to the deduced amino acid sequence of alpha- and beta-subunits of human placenta insulin receptors were used as the first sera. Our results have shown that 30 min after intraperitoneal and intraventricular injections of I U insulin in controls and MSG-treated animals the staining of DAB reaction products in livers became stronger as compared to those injected with saline solution. After intraperitoneal and intraventricular injections of insulin the electron-microscopic investigations of rat livers have found the internalization of IR in hepatocytes by interaction of receptors with exo- and endogenous insulin.