Impact of a single insulin treatment (imprinting) applied during liver regeneration on hepatic insulin receptor development, blood glucose level and liver function parameters in adult rats
Dobozy, O.; Csaba, G.; Inczefi-Gonda, A.; Büki, B.; Németh, J.; Karabélyos, C.
Acta Physiologica Hungarica 79(3): 331-338
1992
ISSN/ISBN: 0231-424X PMID: 1340089 Document Number: 394681
Male Wistar rats weighing 250 g subjected to partial hepatectomy (surgical removal of two-thirds of the liver) showed no appreciable change in serum cholesterol, bilirubin, albumin, total protein and albumin:globulin ratio values at 2, 5, 12 and 21 days after the intervention. Enzyme activities characteristic of liver damage (aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, alkaline phosphatase) were high in control and low in the insulin-imprinted group at 2 days, tended to become normal in both groups at 5 days and changed slightly at 12 days. Blood glucose was markedly decreased in controls and to a lesser degree also in the experimental group at 2 and 5 days of sampling. Insulin treatment (loading) at 2 and 5 days accounted for a drop of blood glucose which was followed by a return to normal within 2 h. Starving value and response to insulin loading were within the physiological range at 21 days, whereas at 12 days no normalization occurred in either group within 2 h of insulin loading, although the starving value was physiological. Binding capacity of the insulin receptor was low in controls for up to 12 days and tended to return to normal by 21 days. In the insulin-imprinted group, binding capacity increased over the control at 2 and 5 days and became normal by 12 days.