Influence of diet with a high starch or sucrose content on glucose tolerance, serum insulin level and insulin sensitivity in rats
Vrána, A.; Splabochová, Z.; Fábry, P.; Kazdová, I.
Physiologia Bohemoslovaca 23(4): 305-310
1974
ISSN/ISBN: 0369-9463 PMID: 4278142 Document Number: 76493
Female Wistar rats of 150 to 180 g were given for 6 to 10 weeks diets with protein 20, fat 10 and carbohydrate 70% of total energy. The carbohydrate was wheat starch or sucrose. No difference in bodyweight was found between starch and sucrose groups. After the rats had been starved for 16 to 18 h they were given glucose by mouth. Mean serum glucose concentration in rats given the sucrose diet increased from 92.4 to a maximum of 161.6 mg/100 ml at 60 min after glucose loading. In rats given the starch diet mean serum glucose increased from 99.6 to a maximum of 149 mg/100 ml at 30 min. Mean serum insulin increased from 22 to 38.5 mu U/ml at 60 min in the sucrose-fed and from 23 to 33.6 at 60 min in the starch-fed rats. In a second experiment glucose was given intraperitoneally. Serum glucose increased from 96.2 to 146.3 mg/100 ml by 30 min in the starch-fed and from 91.3 to 186.6 in the sucrose-fed rats. Serum insulin increased after 30 min from 22.8 to 89.6 in the sucrose-fed and from 18.7 to 43.9 mu U/ml in the starch fed rats. After intraperitoneal tolbutamide serum glucose decreased from 68.9 to 46.3 by 60 min in the sucrose-fed and from 75.6 to 44.9 mg/100 ml after 30 min in the starch fed rats. Serum insulin increased from 14.9 to 45.8 by 30 min in the sucrose-fed and from 16.7 to 40.1 mu U/ml by 30 min in the starch-fed rats.