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A time-resolved fluorescence anisotropy study of bilayer membranes containing alpha-tocopherol

Bisby, R.H.; Birch, D.J.

Biochemical and Biophysical Research Communications 158(2): 386-391

1989

ISSN/ISBN: 0006-291X
PMID: 2916987
Document Number: 334469
Rotational mobility in fluid phase dipalmitoylphosphatidylcholine unilamellar vesicles containing alpha-tocopherol has been studied by time-resolved anisotropy measurements of fluorescence from a diphenylhexatriene-phosphatidylcholine conjugate. The results are analysed using a simple wobbling-in-cone model. The diphenylhexatriene probe shows an increasing order parameter and more restricted wobbling with increasing alpha-tocopherol content of the membrane. The diffusional rate for wobbling was found not to change significantly.

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