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A technique for rapid purification of low yield products: biotinylation of chemically synthesized proteins on-resin

Deibel, M.R.; Lobl, T.J.; Yem, A.W.

Peptide Research 2(2): 189-194

1989

ISSN/ISBN: 1040-5704
PMID: 2520757
Document Number: 334103
We report here straightforward methodology for the purification of chemically synthesized proteins which are produced in low yield. The methodology is generally applicable to all proteins still on-resin and fully protected except for the terminal amino group. The protein is treated in order with the following steps: Biotinylation with NHS-biotin, HF cleavage, and avidin-agarose affinity chromatography. No special skills or automated equipment are needed to take advantage of this procedure.

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