Effect of rifampicin and RNA polymerase changing mutation on the spectrum of proteins synthesized in Escherichia coli cells
Gorlenko AhM; Afanas'ev, V.M.; Iarulin, V.R.
Molekuliarnaia Biologiia 13(6): 1350-1359
1979
ISSN/ISBN: 0026-8984 PMID: 398002 Document Number: 145922
Rifampicin (30 mkg/ml) drastically changes the spectra of proteins synthesized by E. coli cells. The formation of some polypeptides is stimulated while that of others is inhibited. Thus, the earlier reported rifampicin stimulation of the synthesis of RNA polymerase .beta. and .beta.'-polypeptides is not an exception, the formation of some other proteins is being also enhanced by the antibiotic. After infection of UV-irradiated cells by .lambda.rifd47 transducing phage, the viral proteins are less inhibited than the bacterial ones encoded by the phage. The spectra of proteins synthesized are also affected by rpoC1 mutation at non-permissive temperature. The obtained data suggest that rifampicin and rpoC1 mutation change the interaction of RNA polymerase with different promoters and/or regulatory factors.