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Intergeneric conjugational crossing of Escherichia coli with Salmonella typhimurium. II. Transfer of a polA1 mutation from Escherichia coli to Salmonella typhimurium and its phenotypic expression in the salmonella genome

Stepanova, N.F.; Andreeva, I.V.; Kiriushkina, A.A.; Kondrat'ev, I.S.; Likhoded, L.I.

Genetika 13(12): 2189-2194

1977

ISSN/ISBN: 0016-6758
PMID: 355054
Document Number: 117653
The Escherichia coli structural gene for DNA polymerase I was inserted into Salmonella typhimurium chromosome by conjugal transfer. The genetic analysis of P1-mediated transduction of obtained hybrid showed that polA gene is located in it between metE and rha loci and is cotransduced with metE (about 50%) and rha (12%). The phenotypic properties of polA1 hybrid E. coliXS. typhimurium concerning UV-MMS-NG and gamma-ray sensitivity are similar to the polA1 mutants of E. coli.

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