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Effects of selected muscarinic cholinergic antagonists on [3H]acetylcholine release from rat hippocampal slices

Pohorecki, R.; Head, R.; Domino, E.F.

Journal of Pharmacology and Experimental Therapeutics 244(1): 213-217

1988

ISSN/ISBN: 0022-3565
PMID: 3335998
Document Number: 326419
A number of cholinergic muscarinic (M) agonists and antagonists were studied for their ability to enhance tritiated acetylcholine ([3H]ACh) release from electrically field-stimulated rat hippocampal slices. A Ca++-free medium and carbachol, but not nicotine, inhibited [3H]ACh release. Atropine, methylatropine and dexetimide produced concentration-dependent increases in [3H]ACh release to a maximum of about 50% above control. Aprophen and benactyzine produced a maximal response 25 to 35% above control. The selective M1 antagonist pirenzepine had the least effect on [3H]ACh release. Of the nonspecific M1-M2 antagonists studied, benactyzine produced the least amount of [3H]ACh release. The order of potency of the M antagonists in promoting a 15% increase in [3H]ACh release was aprophen greater than benactyzine greater than methylatropine greater than dexetimide greater than pirenzepine greater than atropine. However, the order of promoting maximal release of [3H]ACh was atropine greater than dexetimide greater than methylatropine greater than aprophen greater than benactyzine greater than pirenzepine.

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Lee, H.J.; Cozzi, N.V.; Weiler, M.H. 1991: Age-related differences in the effects of some muscarinic agents on acetylcholine release from rat neostriatal slices Journal of Pharmacology and Experimental Therapeutics 258(2): 496-501
Scherber, A.; Staib, A.H.; Oelszner, W. 1975: Effect of various amino acids and their antagonists on the cholinergic tremor and acetylcholine release in the CNS of the rat Acta Biologica et Medica Germanica 34(9): 1517-1524
Zhang, Y.F.; Zhang, C.C. 1991: Effects of cholinergic agonists and antagonists on unit discharge of dorsal hippocampal CA1 area in rat Zhongguo Yao Li Xue Bao 12(6): 510-513
Maire, J.C.; Wurtman, R.J. 1985: Effects of electrical stimulation and choline availability on the release and contents of acetylcholine and choline in superfused slices from rat striatum Journal de Physiologie 80(3): 189-195
Pascual-Alonso, T.; González-Zárate, J.L. 1992: Subtypes of muscarinic acetylcholine receptor following the experimental denervation of the cholinergic pathway ascending to the neocortex Archivos de Neurobiologia 55(3): 116-123
Chebabo, S.R.; Santos, M.D.; Albuquerque, E.X. 1999: The organophosphate sarin, at low concentrations, inhibits the evoked release of GABA in rat hippocampal slices Neurotoxicology 20(6): 871-882
Murugaiah, K.D.; O'Donnell, J.M. 1995: Beta adrenergic receptors facilitate norepinephrine release from rat hypothalamic and hippocampal slices Research Communications in Molecular Pathology and Pharmacology 90(2): 179-190
Huang, H.Y.; Dai, S.P.; Xie, P.G. 1992: Modulation of 3,4-diaminopyridine-evoked norepinephrine release from rat hippocampal slices by activation of alpha 2-adrenoceptor Zhongguo Yao Li Xue Bao 13(6): 509-512
Vizi, E.S.; Ono, K.; Adam-Vizi, V.; Duncalf, D.; Földes, F.F. 1984: Presynaptic inhibitory effect of Met-enkephalin on [14C] acetylcholine release from the myenteric plexus and its interaction with muscarinic negative feedback inhibition Journal of Pharmacology and Experimental Therapeutics 230(2): 493-499
Baud, P.; Arbilla, S.; Cantrill, R.C.; Scatton, B.; Langer, S.Z. 1985: Trace amines inhibit the electrically evoked release of [3H]acetylcholine from slices of rat striatum in the presence of pargyline: similarities between beta-phenylethylamine and amphetamine Journal of Pharmacology and Experimental Therapeutics 235(1): 220-229
Paul, V. 1987: Effect of some beta-adrenoceptor antagonists on cerebrocortical acetylcholine release in rats Indian Journal of Medical Research 86: 260-263
Morimoto, Y. 1993: Effects of acidosis on the neuronal function following oxygen-glucose deprivation in the rat hippocampal slices Hokkaido Journal of Medical Science 68(5): 717-727
Grill, V.; Fåk, K. 1985: Influence of thiol groups, calcium, and glucose metabolism on cholinergic-induced insulin release and on methylscopolamine binding to muscarinic receptors in pancreatic islets of the rat Acta Endocrinologica 109(3): 355-360
Salińska, E.; Lazarewicz, J.W. 1994: NMDA receptors mediate anoxia/aglycemia-induced release of eicosanoids in immature rat hippocampal slices: utility of an in vitro "ischemic" superfusion model with temporary arrest of medium flow Folia Neuropathologica 32(3): 155-159
Stasiewicz, J.; Rajkowska-Franke, E.; Kinalska, I.; Gabryelewicz, A. 1980: Effect of adrenergic agonists and antagonists on the pancreatic enzyme release in conditions of cholinergic stimulation (studies in vitro) Przeglad Lekarski 37(12): 791-795
Liang, D.; Xie, Y.; Chai, H. 1992: Effects of adrenergic agonist on population spike in CA1 region of hippocampal slices from partial and full kindled rats Hua Xi Yi Ke Da Xue Xue Bao 23(3): 305-308
Wemer, J.; van der Lugt, J.C.; de Langen, C.D.; Mulder, A.H. 1979: On the capacity of presynaptic alpha receptors to modulate norepinephrine release from slices of rat neocortex and the affinity of some agonists and antagonists for these receptors Journal of Pharmacology and Experimental Therapeutics 211(3): 445-451
Wess, J.; Gdula, D.; Brann, M.R. 1992: Structural basis of the subtype selectivity of muscarinic antagonists: a study with chimeric m2/m5 muscarinic receptors Molecular Pharmacology 41(2): 369-374
Nilius, B. 1983: Dependence of muscarinic effects of acetylcholine in mammalian atrial myocardium on the external calcium and potassium concentration Biomedica Biochimica Acta 42(5): 511-518
Valcana, T.; Liao, C.; Timiras, P.S. 1974: Effects of X-radiation on the development of the cholinergic system of the rat brain. II. Investigation of alterations in acetylcholine content Environmental Physiology and Biochemistry 4(2): 58-63