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Rapid quantification of apolipoprotein E enriched very-low-density lipoproteins by heparin-Sepharose chromatography

Weisweiler, P.

Journal of Chromatography 425(1): 169-174

1988

ISSN/ISBN: 0021-9673
PMID: 3360867
Document Number: 326066
Small samples of human very-low-density lipoproteins (VLDL) were easily separated into 2 distinct subfractions by affinity chromatography on heparin-Sepharose. The high resolving power was combined with a very high reproducibility and linearity of response over an adequate concentration range. The chemical composition and the apolipoprotein distribution of the VLDL subfractions confirm the results of previous investigators that cholesteryl ester and apolipoprotein E are in particularly high concentrations in the bound subfraction. The estimation of cholesterol by a sensitive method allows the direct quantification of this VLDL subfraction.

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