A rapid method for continuous flow measurement of cholesterol contained in high density lipoproteins (HDL)
Ponsot, P.; Yvert, J.P.; Chevrier, M.; Bon, R.
Annales de Biologie Clinique 39(5): 289-293
1981
ISSN/ISBN: 0003-3898 PMID: 7316254 Document Number: 180637
A reagent containing concanavalin A, a plant lectin, was used to selectively precipitate human serum lipoproteins containing apoprotein B, a component of VLDL very low density lipoprotein, LDL low density lipoprotein and lipoprotein (a) which are well known for their atherogenic risk. During this precipitation true high density lipoproteins [HDL] remain in solution. HDL cholesterol determination which constitutes an indirect indication of HDL activity or concentration is performed by an enzymatic method using an automated continuous flow technique carried out on an Auto Analyzer II. This rapid, easy determination obtains results comparable to other methods, particularly those chosen by the French Society of Clinical Biology. This technique should permit all laboratories to confirm an atherogenic index.