Erythrocyte D-glucose transport activity in reconstituted model membranes of different lipid composition
Cestaro, B.; Cervato, G.; Carandente, O.; Girardi, A.M.; Pozza, G.
Biochemistry International 16(2): 323-329
1988
ISSN/ISBN: 0158-5231 PMID: 3365265 Document Number: 321979
The stereospecific influx of D-glucose into liposomes formed on sonication of different glyco- and phospholipids with transport proteins from human erythrocyte ghosts solubilized with Triton x-100 was measured as an index of their total D-glucose transport activity. Specific D-glucose transport increased when acidic phospho- and glycolipids (especially sulfatide) were added to the phosphatidylcholine bilayers of the model membranes while cholesterol strongly inhibited the process. The modulation of D-glucose transport activity and its possible correlation with the lipid composition and the chemico-physical state of the erythrocytes is discussed.