Simultaneous determination of carbamazepine and its epoxide and transdiol metabolites in plasma by microbore liquid chromatography
Riad, L.E.; Sawchuk, R.J.
Clinical Chemistry 34(9): 1863-1866
1988
ISSN/ISBN: 0009-9147 PMID: 3416433 Document Number: 312134
Simultaneous monitoring of carbamazepine and its metabolities is helpful in providing information on the induction process via the epoxide-diol route. The present method involves the liquid-chromatographic analysis of 1.0 mL of plasma for carbamazepine at concentrations of 0.025 to 4.0 mg/L and for both the 10,11-epoxide and 10,11-transdiol metabolites at concentrations of 0.01 to 1.0 mg/L. Weighted regression equations, expressing peak area ratios as a function of concentrations of carbamazepine and its epoxide and transdiol metabolites in the standards, were used to determine concentrations in plasma samples.