Relationship between EB virus and nasopharyngeal carcinoma. I. Detection of EBV DNA-related sequences in biopsy specimens of nasopharyngeal carcinoma
Cui, H.Y.
Zhonghua Zhong Liu Za Zhi 10(4): 260-262
1988
ISSN/ISBN: 0253-3766 PMID: 2854777 Document Number: 308882
Assay of biopsy materials of nasopharyngeal carcinoma (NPC) by nucleic hybridization (dot blot) technique with 32P labelled EBVDNA BamHIW fragment probe (specific radioactivity: 1-3 x 10(7) cpm/micrograms DNA) are described. 57 biopsies of different pathologic types were taken from NPC patients from Beijing and Qing Dao, Shandong province, and 54 control biopsies were taken from patients with other tumors and inflammatory tissues from the nasopharynx and head-neck regions. It was shown that EBVDNA positive reaction was found in 52 out of 57 NPC biopsies (91%), while only 10 positive reaction were observed in 54 control samples (18.5%). There was a significant statistical difference therein (P less than 0.001), but no significant difference of EBV DNA positive rates was found among the different pathological types (P greater than 0.05). Also no significant difference was found in biopsies taken from various geographical regions in both groups.