Immunoenzyme analysis using paper disks
Levi, M.I.; Iagovkina, V.N.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 1988(12): 94-99
1988
ISSN/ISBN: 0372-9311 PMID: 3072810 Document Number: 306683
Filter paper discs have been used in the enzyme immunoassay (EIA) as solid phase instead of polystyrene plates. The use of paper discs has made it possible to achieve a multiple increase in the sensitivity of sandwich EIA, thus permitting the detection of Yersinia pestis capsular antigen at a concentration of 0.4 ng/ml. Paper discs can be used not only for the sorption of antigen and antibodies, but also for the affinity purification of preparations.