High-pressure liquid chromatography analysis of antibiotic susceptibility disks
Hagel, R.B.; Waysek, E.H.; Cort, W.M.
Antimicrobial Agents and Chemotherapy 16(3): 372-377
1979
ISSN/ISBN: 0066-4804 PMID: 507793 Document Number: 150622
Analysis of antibiotic susceptibility disks by high-pressure liquid chromatography (HPLC) was investigated. Methods for potency determination of mecillinam, ampicillin, carbenicillin and cephalothin alone and in various combinations were presented. There was good agreement between HPLC and microbiological data for potency determinations with over 95% recovery. Relative SD lower than 2% were recorded for each HPLC method. HPLC methods offer improved accuracy and greater precision compared to standard microbiological analysis methods for susceptibility disks.