Medrogestone: analysis in serum by high-pressure liquid chromatography
Robinson, W.T.; Cosyns, L.
Arzneimittel-Forschung 29(6): 882-885
1979
ISSN/ISBN: 0004-4172 PMID: 582778 Document Number: 152803
A method for the determination of 6,17-dimethylpregna-4,6-diene-3,20-dione (medrogestone) in serum is described using high-pressure liquid chromatography with UV detection. Interfering substances in human or dog serum blanks were removed selectively by reaction with oxalyl chloride followed by an aqueous alkaline borate "wash" of the extract. The method is specific for medrogestone and, based on 5 ml serum, has a limit of detection of 2 ng/ml.
Document emailed within 1 workday
Related Documents
Ficarra, P.; Ficarra, R.; Tommasini, A.; de Pasquale, A.; Guarniera Fenech, C.; Iauk, L. 1984: High-performance liquid chromatography of flavonoids in Crataegus oxyacantha L. I. Reversed-phase high-pressure liquid chromatography Il Farmaco; Edizione Pratica 39(5): 148-157Iverson, J.L.; Bueno, M.P. 1981: Evaluation of high pressure liquid chromatography and gas-liquid chromatography for quantitative determination of sugars in foods Journal - Association of Official Analytical Chemists 64(1): 139-143
Attina, M.; Ciranni, G. 1977: Use of high pressure liquid chromatography for the analysis of various coloring agents Il Farmaco; Edizione Pratica 32(4): 186-191
Date, C.; Tanaka, H.; Yoshikawa, K.; Baba, T.; Hayashi, M.; Owada, K.; Okazaki, K.; Nakayama, Y.; Ishii, R. 1982: Determination of sugars in daily foods by high pressure liquid chromatography and gas liquid chromatography Osaka City Medical Journal 28(2): 67-83
Cavina, G.; Moretti, G.; Gallinella, B.; Alimenti, R.; Barchiesi, R. 1978: Analysis of corticosteroids in compound pharmaceutical forms using high liquid pressure chromatography: analysis of preparations for dermatological use Bollettino Chimico Farmaceutico 117(9): 534-544
Hagel, R.B.; Waysek, E.H.; Cort, W.M. 1979: High-pressure liquid chromatography analysis of antibiotic susceptibility disks Antimicrobial Agents and Chemotherapy 16(3): 372-377
Xu, X.R.; Xu, H.; Lu, Y.A.; Ghen, J. 1979: Determination of partition coefficient by high-pressure liquid chromatography. I. Determination of partition coefficient of cinnamic acids and related compounds by reversed-phase high-pressure liquid chromatography Yao Xue Xue Bao 14(4): 246-252
Cruz Igartúa, A.; Benítez, F.; Pérez-Cruet, J.; Cancio, M. 1985: Correlation between serum levels of amitriptyline and the Hamilton scale of depression using high-pressure liquid chromatography Boletin de la Asociacion Medica de Puerto Rico 77(9): 375-380
La Rotonda, M.I.; Schettino, O. 1978: Analysis of meglumine in pharmaceutical products by means of high pressure liquid chromatography Bollettino della Societa Italiana di Biologia Sperimentale 54(24): 2590-2594
Lorenzo, B.; Drayer, D.E. 1981: Improved method for the measurement of cimetidine in human serum by reverse-phase high-pressure liquid chromatography Journal of Laboratory and Clinical Medicine 97(4): 545-550
Paczkowski, D.; Dabrowski, R.; Sitkiewicz, D.; Sadowski, Z. 1995: Analysis of diltiazem and desacetyldiltiazem in serum by high-performance liquid chromatography Polish Journal of Pharmacology 47(5): 429-434
Buratti, M.; Pellegrino, O.; Caravelli, G.; Xaiz, D.; Valla, C.; Colombi, A. 1989: Application of high-pressure liquid chromatography in the analysis of urinary metabolites of aromatic solvents La Medicina del Lavoro 80(3): 254-263
Ullman, M.D.; McCluer, R.H. 1987: High-pressure liquid chromatography analysis of neutral glycosphingolipids: perbenzoylated mono-, di-, tri-, and tetraglycosylceramides Methods in Enzymology 138: 117-125
Tojo, Y. 1981: Change of C19-21 steroids levels of maternal serum, amnion and cord plasma by high pressure liquid chromatography (HPLC) Nihon Sanka Fujinka Gakkai Zasshi 33(1): 117-126
MacDougall, M.L.; Shoeman, D.W.; Azarnoff, D.L. 1975: Separation and quantitative analysis of furosemide and 4-chloro-5-sulfamoylanthranilic adid (CSA) by high pressure liquid chromatography Research Communications in Chemical Pathology and Pharmacology 10(2): 285-292
Brown, P.R.; Krstulović, A.M.; Hartwick, R.A. 1977: Analysis of purines and their nucleosides by the reverse phase partition mode of high pressure liquid chromatography Advances in Experimental Medicine and Biology 76a: 610-618
Kliushnichenko, V.E.; Iakimov, S.A.; Arutiunian, A.M.; Ivanov, A.E.; Mal'tsev, K.V.; Vul'fson, A.N. 1994: Genetic engineering of human insulin. IV. Development and optimization of an analysis system using reversed phase high pressure liquid chromatography Bioorganicheskaia Khimiia 20(10): 1080-1088
Sting, R.; Schmitt, M.; Blobel, H. 1989: Isolation of Streptococcus pyogenes fibronectin receptors by affinity chromatography and high pressure liquid chromatography Journal of Chromatography 497: 258-262
Thienpont, L.M.; Verhaeghe, P.G.; Van Brussel, K.A.; De Leenheer, A.P. 1988: Estradiol-17 beta quantified in serum by isotope dilution-gas chromatography-mass spectrometry: reversed-phase C18 high-performance liquid chromatography compared with immuno-affinity chromatography for sample pretreatment Clinical Chemistry 34(10): 2066-2069
Peat, M.A.; Kopjak, L. 1979: The screening and quantitation of diazepam, flurazepam, chloridazepoxide, and their metabolites in blood and plasma by electron-capture gas chromatography and high pressure liquid chromatography Journal of Forensic Sciences 24(1): 46-54