Analysis of diltiazem and desacetyldiltiazem in serum by high-performance liquid chromatography
Paczkowski, D.; Dabrowski, R.; Sitkiewicz, D.; Sadowski, Z.
Polish Journal of Pharmacology 47(5): 429-434
1995
ISSN/ISBN: 1230-6002 PMID: 8868135 Document Number: 449126
This paper describes a simple high-performance liquid chromatographic (HPLC) method for the determination of diltiazem and desacetyldiltiazem in human serum. After basic methyl-tert-butyl ether extraction and back-extraction with hydrochloric acid, the drug and its metabolite was injected into a Supelcosil LC-CN column and the absorbance of the eluate was measured at 240 nm. The sensitivity was 5 ng/ml and the obtained precision, selectivity and stability during storage were adequate for the performed clinical studies in patients therapeutically treated with diltiazem.