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Experimental conditions of a technic of in vitro transcription using placental nuclei

Hess, K.; el Ouardirhi, H.; Belleville, F.; Paysant, P.; Nabet, P.

Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 182(3): 286-295

1988

ISSN/ISBN: 0037-9026
PMID: 2976296
Document Number: 306314
To examine the expression of human placental lactogen, we investigated transcription in isolated placental nuclei. Using SH-agarose affinity chromatography, we have isolated Hg-RNA synthetized in vitro. These RNA are translated in a wheat germ cell-free system. hPL synthetized in vitro, accounting for 20% of total protein. This system should prove useful for the study of gene expression of hPL.

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