DNA-dependent RNA-polymerase Ii from human placental nuclei
Sedova, V.M.; Vorob'ev, V.I.
Biokhimiia 58(10): 1580-1586
1993
ISSN/ISBN: 0320-9725 PMID: 8268301 Document Number: 409254
The procedure for isolation and purification of RNA polymerase II from human placenta nuclei is described. The sensitivity of the enzyme to alpha-amanitin, bivalent cations, ionic strength and glycerol concentration in vitro has been studied. Eleven subunits of the RNA polymerase II molecule have been identified by gel electrophoresis. An optimal RNA polymerase II assay mixture has been developed.
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