Behavior of pericentromere heterochromatin regions under different conditions of chromosome decondensation in isolated nuclei
Stefanova, E.V.; Chentsov, I.S.
Molekuliarnaia Biologiia 24(2): 506-513
1990
ISSN/ISBN: 0026-8984 PMID: 1694570 Document Number: 354895
In isolated mouse nuclei the chromocenters were shown to be the pericentromeric heterodchromatin regions (PCHR). After the decreasing of bivalent ion concentration (0.1 mM Ca2+, 2 mM Mg2+) the main and peripheral parts of the chromatin remained on the contrary as the compact chromatin bodies. The additional ultrasound treatment of isolated nuclei in the presence of 0.1 mM Ca2+ with DNAse II and triton X-100 resulted in the species enriched by the condensed PCHR.